Journal: Science Advances
Article Title: Progressive alterations in amino acid and lipid metabolism correlate with peripheral neuropathy in Polg D257A mice
doi: 10.1126/sciadv.abj4077
Figure Lengend Snippet: ( A ) Schematic of major plasma lipids. ( B ) Concentration of plasma cholesterol in WT and Polg mice. Concentration of total ( C ) and individual ( D ) plasma fatty acids in WT and Polg mice. Abundance of plasma triacylglycerols ( E ) and diacylglycerols ( F ), phosphatidylethanolamine (PE) ( G ), and phosphatidylcholine (PC) ( H ) relative to internal standard per microliter of plasma in WT and Polg mice. Concentration of plasma dihydroceramide ( I ), ceramide ( J ), sphingomyelin ( K ), glucosylceramide ( L ), and lactosylceramide ( M ) in WT and Polg mice. Two-sided Student’s t test for each comparison with no adjustment for multiple comparisons. Data are means ± SEM of n = 7 to 8 animals per group. * P < 0.05, ** P < 0.01, and **** P < 0.0001.
Article Snippet: For targeted sphingolipid analysis, 50 μl of plasma was extracted with 500 μl of −20°C methanol, 400 μl of saline, and 100 μl of water spiked with deuterated internal standards [100 pmol of D7-sphingosine (Avanti, Croda International Plc, 860657), 20 pmol of D7-sphinganine (Avanti, Croda International Plc, 860658), 2 pmol of D3-deoxysphinganine (Avanti, Croda International Plc, 860474), 200 pmol of C15 ceramide-d7 (d18:1-d7/15:0) (Avanti, Croda International Plc, 860681), 100 pmol of C13-dihydroceramide-d7 (d18:0-d7/13:0) (Avanti, Croda International Plc, 330726), 10 pmol of glucosylsphingosine (d18:1-d7) (Avanti, Croda International Plc, 860695), 100 pmol of glucosylceramide (d18:1-d7/15:0) (Avanti, Croda International Plc, 330729), 100 pmol of lactosylceramide (d18:1-d7/15:0) (Avanti, Croda International Plc, 330727), and 200 pmol of sphingomyelin (d18:1/18:1-d9) (Avanti, Croda International Plc, 791649)].
Techniques: Concentration Assay